TABLE 1.
Mutations identified in infectious J/C-NS2H77 and J/C-NS3proH77-Ubi pseudorevertants rescued following passage of transfected cells
| Protein | Amino acid substitution(s) ina: |
||||||
|---|---|---|---|---|---|---|---|
| J/C-NS2H77 virus (day posttransfection): |
J/C-NS3proH77-Ubi virus (day posttransfection): |
||||||
| 1 (30) | 2 (65) | 3 (50) | 4 (35) | 1 (40) | 2 (65) | 4 (35) | |
| E1H77 | I348T | M345T | M345T | M345T | |||
| E2H77 | E591G | E591D | Y718S | ||||
| Ubi | I44T | ||||||
| NS3JFH1 | Q1251L | R1412W | Q1251L | Q1251L | P1032L, Q1251L | P1159Q | S1328T, R1412W |
| NS5AJFH1 | N2156K | S2358R | L2276P | ||||
| NS5BJFH1 | S2552G | S2457G | |||||
a
Amino acid numbering corresponds to amino acid position within H77 polyprotein, ubiquitin (Ubi), or JFH1 polyprotein, as indicated in the protein name. Amino acid substitutions found in several viruses (viruses 1 to 4) rescued independently at the indicated numbers of days posttransfection are represented in bold characters. Substitutions present in 100% of bulk virus populations are highlighted by gray shading.