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. 2015 Sep 23;89(23):12178–12188. doi: 10.1128/JVI.01757-15

FIG 7.

FIG 7

Characterization of Elijah cells infected with the ΔLMP-1 or wt EBV. (A) Western blot analysis of LMP-1 and EBNA2 expression in Elijah cells infected with the LMP-1 mutant or wt virus. Actin was used as a loading control. (B) RT-qPCR analysis of miR-146a and miR-155 expression in Elijah cells infected with either an LMP-1 knockout virus (ΔLMP-1) or wt EBV B95.8. (C) RT-qPCR analysis of miR-182 and miR-183 expression in the same cells. Results of miRNA RT-qPCR were normalized to U6 snRNA expression. (D) Quantification of the expression of the pri-mir-183-96-182 primary transcript by RT-qPCR after normalization to GAPDH in cells infected with the EBV ΔLMP-1 mutant or the wt virus. (E) RT-qPCR quantification of EBV–miR–BHRF1-2 and -BART22 in the same cells. U6 snRNA was used as a normalizer. *, P < 0.01 (Student t test).