FIG 9.

Role of Akt signaling in LMP-1-induced regulation of the miR-183-96-182 cluster. Mutu-III, Kem-III, Sav*, and BL41-B95.8 cells were treated with Akt inhibitor X (Akt inh X) for 48 h. (A) Effect of Akt inhibition on miR-96 expression in the indicated cell lines. Results were normalized to U6 snRNA expression. Ctr, control. (B) RT-qPCR quantification of KRAS mRNA expression in the same cell lines treated with the Akt inhibitor or left untreated. Transcript levels were normalized to GAPDH expression. *, P < 0.05; **, P < 0.01 (Student t test).