FIG 1.

Identification of GAPDH as an iNS1-interacting partner. (A) Extracts from mock-infected (Mock) and DENV2-infected (DENV) HUVEC-C cells were added to a column containing immobilized anti-NS1, and the eluted fractions were separated by SDS-PAGE prior to band excision and in-gel digestion. (B) The trypsinized peptides were subjected to Q-Tof MS/MS analysis under both mock infection and DENV infection conditions. MM, molecular mass. (C) The peak corresponding to the most abundant peptide was identified and selected for a second round of MS. The peptide sequence (with z being equal to 2) was then analyzed by use of the Mascot algorithm, using the sequences in the Swiss-Prot database for protein identification. The identified peptide corresponded to a unique fragment of the GAPDH protein, which was confirmed by a search of the NCBI database. The results presented here are representative of those from two independent experiments.