FIG 3.

Confirmation of the NS1-GAPDH interaction by a cross-linking assay using the cross-linking reagent EGS. Different amounts of the rNS1 (1.5 or 3 μg) and GAPDH (3, 4, or 6 μg) proteins were incubated in the absence or presence of 10 mM EGS. The samples were incubated for 30 min at room temperature prior to analysis by Western blotting using anti-GAPDH (A) or anti-NS1 (B) antibodies. As a control, both GAPDH (6 μg) and rNS1 (3 μg) were incubated alone in either the absence or the presence of 10 mM EGS, followed by Western blot analysis with their respective antibodies. The results presented here are representative of those from two independent experiments.