FIG 3.

Virus titer in nasal washes, lungs, and nasal turbinates of guinea pigs exposed to bovine FLUDV by the aerosol or contact route. In both the contact transmission group and aerosol transmission group, 3 guinea pigs were intranasally inoculated with 3 × 10⁵ TCID₅₀/300 μl of bovine FLUDV. After 24 h of infection, 3 uninfected guinea pigs were added to each cage as sentinels. Nasal washes were collected at days 2, 4, 6, 8, 10, 12, and 14 dpi from all animals. All the guinea pigs were euthanized at 14 dpi. (A and C) Virus titers in nasal washes from the contact transmission group (A) and the aerosol transmission group (C) are expressed as log₁₀ TCID₅₀ per milliliter. (B and D) Virus titers in lungs (B) and nasal turbinates (D) of the infected and sentinel animals in the contact and aerosol transmission groups at 14 dpi are expressed as log₁₀ TCID₅₀ per gram. For panels B and D, each shape represents an individual animal.