TABLE 2.
Viral genome and protein sequence changes in directly inoculated and sentinel contact-infected animal lung isolates compared to parent inoculum of FLUDVa
| Segment | Sequence category | Sequence change(s) |
|
|---|---|---|---|
| Directly inoculated animals | Sentinel infected by contact | ||
| HEF | nt | C755T | A746G |
| aa | A252V (45.2) | N249S (99.1) | |
| P42 | nt | G121A; *A122G; C946T | G121A; *A122G; C946T |
| aa | E41R (98.6); L316F (98.5) | E41R (99.5); L316F (98.5) | |
| NP | nt | G741C; *T760C; *T768C; *A792G | G741C; *T760C; *T768C; *A792G |
| aa | E247D (99.8) | E247D (99.7) | |
| P3 | nt | *A297G; *A804G | *A297G; *A804G |
| aa | |||
| PB1 | nt | *T18G; *T21C | *T18G; *T21C; G694A |
| aa | D232N (52.4) | ||
| PB2 | nt | G132C; C2004G | |
| aa | E44D (94.1); N668K (92.9) | ||
a
Asterisks (*) indicate silent mutations. nt, nucleotide; aa, amino acid. Note that no mutations were observed in the NS segment. Percent frequency of each amino acid mutation is given in parentheses. Unique sequence changes in sentinel infected animals are highlighted in boldface characters. Also note that, because the sequences for the PB1 and PB2 segments were not resolved in high quality in RNA-seq, the PB1 and PB2 sequence changes were calculated based on the sequence of D/660, which was used to produce the parent inoculum used for animal infection experiments.