FIG 2.

No evidence of inheritance of viRNAs after Orsay virus infection. (A) The 23-nt sense (left) Dicer products and 22G antisense (right) secondary RNAs from Fig. 1 normalized to library size and the level in the P0 generation. (B) Percentages of P0 and F1 animals displaying the dpy- or unc-encoded phenotype following exposure to RNAi. Error bars show the standard deviation of three (P0) or four (F1) biological replicates. **, P < 0.005; NS, not significant (t test). (C) The experimental design for the data shown in panel D. (D) qRT-PCR data for the relative levels of Orsay virus 4 days after exposure in animals whose parents were either infected with Orsay virus (+V+V) or uninfected (−V+V) (Orsay virus infection of parents was confirmed by qRT-PCR). Data are normalized to gapdh levels and the level of infection of the +V parents. Error bars show the standard error of the mean. (E) Graph showing a comparison of Orsay virus infection levels between liquid- and agar-based infection protocols in both the drh-1 mutant and N2 strains with three different concentrations of virus. Each condition was performed with five biological replicates, and error bars represent the standard error of the mean.