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Variations on caspase 3 cleavage. (A) Comparison of the caspase 3 cleavage sites of nairovirus-encoded NPs. The stalk domains of the CCHFV, HAZV, KUPV, and ERVEV NPs are aligned and shown in white cartoon diagrams with the same orientation. The residues covering the caspase 3 cleavage sites in each NP are highlighted in different colors. (B) Caspase 3 proteolysis of ERVEV NP and HAZV NP in vitro. (C) Caspase 3 proteolysis of KUPV NP wild type (wt) and E270D mutant in vitro.
