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Thermal stability by DSC. DSC thermograms for WT, WDWD, and ΔLoop are shown as indicated in the panels. Unfolding of the three Fabs shows a single thermal transition between 30 and 90°C. The midpoint of the thermal unfolding (Tm) and the associated enthalpy (ΔH) are also given. The values of Tm and ΔH were obtained by curve fitting with the program ORIGIN 7.0.
