FIG 1.

Construction of the bb0449 mutant (bb0449Δ1-97 and bb0449Δ4-97) and allelic restoration (bb0449^AR) strains. Gene ORFs are represented by gray arrows, and the bb0449 ORF is in dark gray. Primer locations are shown with small black arrowheads and numbers and correspond to Table S1 in the supplemental material. (A) Construction of the bb0449 mutants. The full or partial bb0449 ORF was replaced with the kanamycin antibiotic resistance cassette, flaB_p-kan, from one of the bb0449-knockout vectors (pbb0449KOVΔ1-97 or pbb0449Δ4-97). (B) Closeup of junction between the end of rpoN and the bb0449 deletion-flaB_p-kan insertion region. The BglII-BamHI hybrid site creating the junction is shown in bold type. Strain names are indicated to the left of the sequence. (C) Construction of the bb0449 allelic restoration strain (bb0449^AR). The bb0449 ORF was restored to bb0449Δ4-97 (linked to a downstream gentamicin antibiotic resistance marker, flgB_p-aacC1) by allelic exchange with pbb0449ARV. Figure not drawn to scale.