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Localization of BB0449 protein by immunoblot analysis of fractions during ribosome preparation from WT B. burgdorferi. Equivalent portions of fractions indicated above lanes were separated by SDS-PAGE, blotted, and hybridized with anti-BB0449 antibody. bb0449Δ4-97, total cell lysate equivalent to 108 cells; BB0449, purified recombinant His-tagged protein; kDa, molecular masses of size standards that migrated at indicated positions.
