Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Plasmids used in this study
| Plasmid | Purpose | Strain generated |
|---|---|---|
| pbb0449KOVΔ1-97 | Delete entire bb0449 ORF and insert a kanamycin resistance cassette, truncating rpoN | bb0449Δ1-97 |
| pbb0449KOVΔ4-97 | Delete bb0449 by replacing most of the bb0449 ORF with a kanamycin resistance cassette, leaving rpoN intact | bb0449Δ4-97 |
| pbb0449ARV | Restore bb0449 at its native locus; marked with a gentamicin resistance cassette | bb0449AR |
