FIG 3.

Peripheral blood leukocyte counts. (A and B) Leukocytes isolated from peripheral blood collected at 6 h from control (sham-infected) mice or from those infected with the blood isolate (ST15/4495) or the ear isolate (ST15/9-47) were examined by FACS analysis after staining with anti-Ly-6G (A) or anti-F4/80 (B), as described in Materials and Methods. Data are presented as the product of geometric mean fluorescence intensity and the total number of positive cells (GMFIn) for the respective marker (± SE). (C) Alternatively, blood films were subjected to differential cell counts, and neutrophil numbers are expressed as a percentage of total leukocyte levels (mean ± SE). Statistical significance is indicated as follows: *, P < 0.05; **, P < 0.01 (Student's paired, two-tailed t test).