FIG 1.

Blockade of classical or alternative macrophage polarization does not affect Pneumocystis clearance or development of anti-Pneumocystis antibodies. (A) Pneumocystis burden in the lungs of RAG2^−/−, RAG2/IL-4Rα^−/−, and RAG2/IFN-γR^−/− mice was measured by real-time PCR quantitation of Pneumocystis kexin gene copy numbers in lung homogenates at days 14, 18, 21, 26, and 34 post-immune reconstitution. Values are means ± 1 standard error (n = 6 to 10 per group at each time point). Data represent results from at least two independent experiments. *, P < 0.05 compared to RAG2^−/− mice; #, P < 0.05 compared to RAG2/IL-4Rα^−/− mice. ND indicates that no signal was detected in any mice at that time. Pneumocystis-specific IgG and IgM (B), IgG1 (C), and IgG2a (D) antibodies in Pneumocystis-infected RAG2^−/−, RAG2/IL-4Rα^−/−, and RAG2/IFN-γR^−/− mouse sera were measured by an ELISA using soluble Pneumocystis antigen from infected mouse lung homogenates as the target. OD, optical density. *, P < 0.05 compared to the indicated group.