FIG 2.

Impaired alveolar macrophage polarization in RAG2/IL-4Rα^−/− and RAG2/IFN-γR^−/− mice. (A and B) BAL fluid cells were collected from immune-reconstituted, Pneumocystis-infected RAG2^−/−, RAG2/IL-4Rα^−/−, and RAG2/IFN-γR^−/− mice and stimulated with PMA and ionomycin. Total numbers of CD4⁺ T cells producing IFN-γ and IL-4 were determined by intracellular cytokine staining. Values are means ± 1 standard error (n = 3 to 5 per group at each time point). (C and D) BAL fluid cells were collected from Pneumocystis-infected RAG2^−/−, RAG2/IL-4Rα^−/−, and RAG2/IFN-γR^−/− mice at day 18 post-immune reconstitution. (C) Cells were stained with antibodies specific for the alveolar macrophage marker CD11c (green) and arginase (red). (D) Cells were also stained with antibodies specific for CD11c (red) and iNOS (green). Nuclei were stained with DAPI (blue). At least 200 CD11c⁺ cells were examined for each strain. Magnification, ×400.