FIG 6.

Excessive IFN-γ production impairs the CD8⁺ T cell response during PcP-related IRIS. (A and B) IL-4 (A) and IFN-γ (B) levels in the lung homogenates from immune-reconstituted Pneumocystis-infected RAG2^−/−, RAG2/IL-4Rα^−/−, and RAG2/IFN-γR^−/− mice were measured (n = 6 to 10 per group at each time point). (C) In a separate experiment, Pneumocystis-infected RAG2/IFN-γR^−/− mice were immune reconstituted and treated with either anti-IFN-γ or control rat IgG beginning at day 3 postreconstitution. At day 18 postreconstitution, the mice were sacrificed, and lung CD8⁺ T cell numbers were determined by flow cytometry. Values are means ± 1 standard error (n = 4 to 5 per group at each time point). *, P < 0.05; **, P < 0.01; ***, P < 0.001.