FIG 4.
Interplay between IL-10, P2X7, and Ca2+ in modulating NLRP3 assembly. (a) The expression of ATP-gated P2X7 ion channels was evaluated by Western blotting of lysates collected from Chlamydia-pulsed or nonpulsed WT and IL-10−/− DCs after 0, 1, and 2 h. Note that all samples were run on the same gel and that the figure showing the gel was spliced for better presentation. (b) Intracellular Ca2+ concentrations of Chlamydia-pulsed or nonpulsed WT and IL-10−/− DCs at 0, 1, and 2 h. Data are presented as means ± SDs. *, P ≤ 0.05 using Student's t test to compare the results for Chlamydia-infected WT and IL-10−/− mice. Data from 10 independent experiments were pooled.