TABLE 1.
Oligonucleotide primers used in this study
| Name and purpose | Sequencea (5′→3′) | Gene(s) |
|---|---|---|
| Disruption of comEB | ||
| 599CAKnockfor | CTGAATTCTCAGATTCACAAAAGGGAACAG (EcoRI) | |
| 599EryKnockrev | CAGGATCCTAGGGACCTCTTTAGCTCCTTG (BamHI) | |
| 599tn917for | CAGGATCCCGAAGGATCACTCATGGACTAA (BamHI) | |
| 599CCKnockrev | GCACTGCAGATCTTAGTGCACTTGGTGCAAA (PstI) | |
| Complementation | ||
| ComAF | CAGGATCCGAACAGCACGGCCAATTTAC (BamHI) | comEB |
| ComBR | CTGAATTCTGCAATATAAAACACTTAAATCACGA (EcoRI) | |
| ComAF | CAGGATCCGAACAGCACGGCCAATTTAC (BamHI) | comEB, comEC |
| ComER | CTGAATTCTTTTGAGGCATGCTTATAACTCC (EcoRI) | |
| Real-time PCR | ||
| aroE1F | AACGAGCAGCAATCAATACC | aroE |
| aroE1R | TTCAATAATTCCCCCACCTG | |
| gyrBF | CACACGTATGAAGGCGGAAC | gyrB |
| gyrBR | CTACGGCCGTTAAACCTTCTC | |
| holAF | AAATTGGATTCAGCAACAATTACA | holA |
| holAR | GGTCTATCCCCTATGAACAACATT | |
| atlF | TGCATTTGTACATGCGTTTG | atlL |
| atlR | GCGCAAATGAAGCATAGTCA | |
| aalF | CCTTTACCAATAGCTGCACGA | aal |
| aalR | ACTGGCACAGCTTCAGGTTC | |
| nucF | AAACACGTCCATAGCGATCA | nucI |
| nucR | CGTTGATACGCCTGAAACAG |
a
Restriction sites are underlined.