FIG 6.

The MLN CD11b⁻ CD8⁺ DC subset isolated from infected mice elicits a significant IEL response. LP or MLN DC subsets (CD11b⁺ CD8⁻ or CD11b⁻ CD8⁺ DC) from mice treated as described above were sorted and adoptively transferred via tail vein injection (2.5 × 10⁴ cells/mouse). (A) Gut IEL cytotoxic responses from recipients were assessed 7 days later via expression of CD107a. (B) Activation of the IEL was measured after transfer with CD43 and Ly6C. (C) α4β7 expression by CD8αβ IEL showing newly recruited cells. Graphs depict the frequencies or total numbers of CD8αβ IEL that are CD107a⁺, CD43⁺ Ly6C⁺, or α4β7⁺ cells. All dot plots (A to C) are gated on live CD8αβ IEL. CD8⁺ and CD11b⁺ labels on graphs correspond to CD11b⁻ CD8⁺ and CD11b⁺ CD8⁻ DC, respectively. Data represent results of at least 2 experiments with 3 mice/group.