FIG 3.

Northern blots and qRT-PCR of fnbA. The effect of clonal variation on fnbA expression was assessed by Northern blotting (A) and qRT-PCR (B). (A) Total cellular RNA from S. aureus strains SH1000ΔfnbA (lane 1), CC45 (lanes 2, 3, 4, 5, and 6 for strains 300-169, 301-188, 300-103, 300-087, and 324-136, respectively), and CC5 (lanes 7, 8, 9, 10, and 11 for strains 088-237, 300-246, 088-180, 077-107, and 010-016, respectively). Cells were grown in TSB and isolated at 6 h postinoculation. (B) fnbA transcription by qRT-PCR, normalized by gyrB, at 6 h postinoculation. Isolates marked by an asterisk (*) came from human patients with persistent bacteremia (PB) (strains 300-169, 324-136, 300-246, 088-180, and 077-107), whereas isolates without an asterisk are from human patients with resolving bacteremia (RB). For CC45 versus CC5, a P value of <0.001 was determined with the Student t test.