Figure 2.

AT7519 causes a more pronounced apoptotic response in MYCN-amplified neuroblastoma cells. A, Western Blot analysis of the in vitro effects of AT7519 on Rb phosphorylation, PARP cleavage and MYCN protein expression after 48 h treatment with concentrations equal to the IC₂₅, IC₅₀ or IC₇₅ for each individual cell line (IC₂₅, IC₅₀ and IC₇₅ values are specified in Supplementary Table S1). β-actin was used as household protein. Because of the limited expression of β-actin in neuroblastoma cell line SJNB6, α-tubulin was used as household protein for SJNB6. B, FACS analysis of the in vitro effects of AT7519 on sub-G1 induction after 72 h treatment. *As 72 h treatment of IMR32 cells with 150 nmol/L AT7519 resulted in complete cell degradation, IMR32 cells were treated with 100 nmol/L instead of 150 nmol/L AT7519.