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. 2015 Nov 15;25:10. doi: 10.1186/s12610-015-0026-z

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© Dirami et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunodetection of SLC26A8 and SEPTIN 4 on sperm preparations from individual I1. a, c and e Control semen and b, d and f patient semen. Antibodies SE5362 and L2CL4, directed against SLC26A8, label the annulus of control spermatozoa, but not of spermatozoa from individual I1. The SE5362 antibody correctly detects SLC26A8 at the equatorial segment in spermatozoa from both control and individual I1. SEPTIN 4 is detected at the annulus in spermatozoa from both the control and individual I1. Mitotracker Red 580 was used for MP staining. Bars: 5 μm