FIGURE 1.

TbTim62 is a novel mitochondrial protein in T. brucei. A, schematics of TbTim62 protein. The N-terminal mitochondrial-targeting signal (MTS) is shown in red. Positions of the predicted ankyrin repeat domain and nucleotide binding sites are in brown and orange boxes, respectively. Predicted transmembrane domains (TM1-TM4) are in the blue rectangle. Positions of these TMs are indicated by numbers of the amino acid (AA) residues. B, TbTim62 is localized in mitochondria. T. brucei procyclic form cells expressed TbTim62 with a TAP tag at the C terminus. After induction of expression with doxycycline, cells were stained with sheep IgG antibody followed by FITC-conjugated secondary antibody. Mitotracker and DAPI were used to visualize mitochondria and the nucleus and kinetoplast, respectively. Overlay of the FITC, Mitotracker, and DAPI is shown. DIC, differential interference contrast.