FIGURE 6.

TbTim17 protein decay in wild type control and TbTim62 KD T. brucei. The Wt control and TbTim62 KD cells were grown in the presence of doxycycline for 2 days. Cells were treated with cycloheximide (50 μg/ml) under normal growth conditions. A, at different time points (0–24 h) cells were harvested, and total cellular proteins were analyzed by SDS-PAGE and immunoblot analysis using anti-TbTim17 antibody. Tubulin was used as the loading control. B, intensity of the TbTim17 protein band were quantitated by imaging densitometer, normalized with the intensity of the corresponding tubulin band, and plotted against time. The number of experiments was three. ***, p values <0.001.