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. 2015 Jul 30;290(38):22945–22954. doi: 10.1074/jbc.M115.663450

FIGURE 1.

FIGURE 1.

Characterization of a new Tyr-1070 antibody that recognizes the phospho-Tyr-1070 site of GluN2B and determination of the distribution pattern of Tyr(P)-1070 on GluN2B. A, sequence alignment of GluN2B around the Tyr-1070 residue in different species. B, representative blots of Tyr(P)-1070 levels in rat cortex lysates with or without CIP treatment (n = 3). C, representative blots of Tyr(P)-1070, Tyr(P)-1472, and GluN2B levels in cortical neuron lysates with or without Tyr(P)-1070 peptide (PEP) treatment (n = 3). D, quantification of Tyr(P)-1472 levels in C. NS, no significant difference. E, representative blots of Tyr(P)-1070 levels from GluN1/GFP-GluN2BWT- and GluN1/GFP-GluN2BY1070F-transfected cells assessed by Tyr-1070-specific antibody (n = 3). F, representative blots for Tyr(P)-1070, GluN2B, PSD95, and Fyn levels in different brain regions of adult male rat: mPFC, medial prefrontal cortex; Hippo, hippocampus; Amyg, amygdala, Stria, striatum; Cereb, cerebellum (n = 3). G, representative blots of Tyr(P)-1070 and Fyn levels in P2, non-PSD, and PSD fractions. Enrichment of PSD95 in the PSD fraction indicates successful separation (n = 3).