Skip to main content
. 2015 Jul 30;290(38):22945–22954. doi: 10.1074/jbc.M115.663450

FIGURE 2.

FIGURE 2.

The tyrosine kinase Fyn phosphorylates GluN2B at Tyr-1070. A, the Fyn kinase inhibitors PP2 (10 μm) and Su6656 (2 μm) reduced the Tyr(P)-1070 levels in cultured cortical neurons (DIV 14–16), whereas PP3 did not. B, summary data of panel A (n = 4; **, p ≤ 0.01; NS, no significant difference, one-way analysis of variance). C, immunoblotting revealed that lentivirus-mediated Fyn knockdown reduced Tyr(P)-1070 as well as the Tyr(P)-1472 level in cultured cortical neurons. D, summary data from C (n = 4; **, p ≤ 0.01, two-tailed t test). E, immunoblotting revealed that Tyr(P)-1070 and Tyr(P)-1472 levels were significantly reduced in Fyn knock-out mice compared with wild-type mice. F, summary data from E (n = 3 mice/group, **p ≤ 0.01, two-tailed t test). G, immunoblotting showed that recombinant Fyn dose-dependently increased the Tyr(P)-1472 and Tyr(P)-1070 levels of GluN2B in transfected HEK293 cells. H, summary data from G. All data are expressed as the mean ± S.E.