FIGURE 4.

NcLPMO9C EPR spectra. a, EPR spectra of 160 μm Cu²⁺-loaded NcLPMO9C in the absence (upper panel) or presence (lower panel) of the soluble substrates cellohexaose (20 mg/ml) or xyloglucan (15 mg/ml). Spectra were recorded at 77 K, 1 milliwatt of microwave power, and 10 gauss modulation amplitude. The simulated spectra (SIM) for each species are shown below the corresponding experimental spectra (simulation parameters for the cellohexaose and xyloglucan spectra were identical). b, effect of substrate on super hyperfine splitting in the high field region. The splitting constants (|A| ∼13 × 10⁻⁴ cm) are consistent with coupling to nearby nitrogen nuclei. Spectra were recorded at 77 K, 1 milliwatt of microwave power, and 2 gauss modulation amplitude.