FIGURE 6.

Thermograms. a, upper panels, binding isotherms with theoretical fits (lower panels) obtained for the titration of NcLPMO9C (left) and NcLPMO9C-N (right) into 0.9 μm xyloglucan (top) and 0.146 mg/ml PASC (bottom). The concentration of PASC was set to be 4.5 μm based on an estimated degree of polymerization of 200 (38). All experiments were carried out at pH 5.5. The temperature was 25 °C for PASC and 10 °C for xyloglucan; b, upper panel, binding isotherms with theoretical fits (lower panel) obtained for the binding of 11 mm Glc₆ to 30 μm NcLPMO9C-Cu²⁺ at t = 25 °C.