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Rv3701c encodes for a histidine methyltransferase. The methylation activity of Rv3701c in the presence of AdoMet and histidine was analyzed by ESI-MS. A, Rv3701c catalyzes the methylation of the α-amino nitrogen atom of histidine to form mono-, di-, and trimethylated histidine. B, reaction in the absence of Rv3701c. No methylated histidine products were observed. amu, atomic mass units.
