Skip to main content
. 2015 Jul 16;290(38):23320–23335. doi: 10.1074/jbc.M115.639534

TABLE 1.

Sequences of oligonucleotide primers used for GUS constructs, constructs designed for the heterologous expression of proteins, and quantitative RT-PCR analyses

Primer name Sequence Amplicon size gDNA/cDNA
bp
GUS construction primers
    pPMEI7-F 5′-ACTGGTTCTTCCCAAAAGGTA-3′ 643
    pPMEI7-R 5′-TTTAGATTTAAAACACAAATGGGTTATTTTAG-3′

Heterologous expression construction primers
    PME3-for 5′-ATTCTGCCCGGGATGGCACCATCAATGAAAG-3′ 1779
    PME3-rev 5′-AAAACAATAAGCGGCCGCTAAGACCGAGCGAGAAG-3′
    PMEI7-for 5′-GAGGGATCCAGAAACCTTGAAGAGGAATCAAG-3′ 600
    PMEI7-rev 5′-GAGGTCGACGAAATGATTTCAATGCTTGGAAGC-3′

Quantitative PCR primers
    PME3-qF 5′-TCAAATGTCTCATCGCCGGAACC-3′ 80
    PME3-qR 5′-AGCGTGGATGTCACAGTCTTGG-3′
    PMEI7-qF 5′-TTGTGAGTGTTGCGGAAGAG-3′ 97
    PMEI7-qR 5′-CCCAAAGACTAAACCCCTTTG-3′
    CLA-F 5′-GTTTGGGAGAAGAGCGGTTA-3′ 114
    CLA-R 5′-CTGATGTCACTGAACCTGAACTG-3′
    TIP41-F 5′-GCTCATCGGTACGCTCTTTT-3′ 117
    TIP41-R 5′-TCCATCAGTCAGAGGCTTCC-3′