TABLE 1.
Sequences of oligonucleotide primers used for GUS constructs, constructs designed for the heterologous expression of proteins, and quantitative RT-PCR analyses
Primer name | Sequence | Amplicon size gDNA/cDNA |
---|---|---|
bp | ||
GUS construction primers | ||
pPMEI7-F | 5′-ACTGGTTCTTCCCAAAAGGTA-3′ | 643 |
pPMEI7-R | 5′-TTTAGATTTAAAACACAAATGGGTTATTTTAG-3′ | |
Heterologous expression construction primers | ||
PME3-for | 5′-ATTCTGCCCGGGATGGCACCATCAATGAAAG-3′ | 1779 |
PME3-rev | 5′-AAAACAATAAGCGGCCGCTAAGACCGAGCGAGAAG-3′ | |
PMEI7-for | 5′-GAGGGATCCAGAAACCTTGAAGAGGAATCAAG-3′ | 600 |
PMEI7-rev | 5′-GAGGTCGACGAAATGATTTCAATGCTTGGAAGC-3′ | |
Quantitative PCR primers | ||
PME3-qF | 5′-TCAAATGTCTCATCGCCGGAACC-3′ | 80 |
PME3-qR | 5′-AGCGTGGATGTCACAGTCTTGG-3′ | |
PMEI7-qF | 5′-TTGTGAGTGTTGCGGAAGAG-3′ | 97 |
PMEI7-qR | 5′-CCCAAAGACTAAACCCCTTTG-3′ | |
CLA-F | 5′-GTTTGGGAGAAGAGCGGTTA-3′ | 114 |
CLA-R | 5′-CTGATGTCACTGAACCTGAACTG-3′ | |
TIP41-F | 5′-GCTCATCGGTACGCTCTTTT-3′ | 117 |
TIP41-R | 5′-TCCATCAGTCAGAGGCTTCC-3′ |