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Km and Vmax values of PME3 obtained with HG types containing different degrees and pattern of methylation
PME3-His6 activity assays were performed at pH 7.5 on HG96B82, HG96B69, HG96B20, HG96B77P63, and HG96P64 substrates as described (43).
| Substrate | Apparent Vmax | Apparent Km |
|---|---|---|
| μmol MeOH/min/μg PME3 | μm substrate | |
| HG96B82 | 0.07 | 12 |
| HG96B69 | 0.05 | 5 |
| HG96B20 | 0.10 | 9 |
| HG97B77P63 | 0.05 | 6 |
| HG96P64 | 0.02 | 224 |
