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. 2015 Jul 16;290(38):23320–23335. doi: 10.1074/jbc.M115.639534

TABLE 3.

DB and DM for HG96B82 pretreated with PME3, PMEI, or a PME3/PMEI mixture at pH 6

The preincubated samples were digested by either pectin lyase or endopolygalacturonase. Values are the means of three samples. DB and DBMe (of non-methylesterified zones and methylesterified zones, respectively) are shown. DB and DBMe were determined as described (51). Values are expressed in mol % ± S.D.

DM DB DBMe
mol % mol % mol %
HG96B82 80.5 ± 1.0 3.6 ± 1.8 81.4 ± 3.1
HG96B82 + PME3 13.0 ± 0.6 105.0 ± 6.0 33.8 ± 3.6
HG96B82 + PMEI7 79.4 ± 0.6 1.9 ± 0.1 82.7 ± 3.7
HG96B82 + PME3 + PMEI7 43.2 ± 0.6 89.1 ± 6.9 72.0 ± 2.3