FIGURE 6.

Calixarenes exhibit selectivity for the PHD fingers of JARID1A and MLL1. a, the crystal structure of the JARID1A PHD3 finger in complex with H3K4me3 peptide (PDB code 3GL6). The aromatic cage residues are colored yellow. b, superimposed ¹H,¹⁵N HSQC spectra of JARID1A PHD3, collected as first H3K4me3 peptide and then (1), (4), or (7) were titrated in. The spectra are color-coded according to the protein:peptide:calixarene molar ratio. c, overlays of ¹H,¹⁵N HSQC spectra of the H3K4me2-bound JARID1A PHD3 recorded during titration with (1). d, superimposed ¹H,¹⁵N HSQC spectra of MLL1 PHD3 collected as first H3K4me3 peptide and then (1), (4), and (7) were added gradually. e, the crystal structure of the MLL1 PHD3 finger in complex with H3K4me3 peptide (PDB code 3GL6). The aromatic cage residues are colored blue. f, the normalized chemical shift changes observed in the MLL1 PHD finger upon binding to H3K3me3 (red) and inhibition by (1) or (4) (blue) as a function of residue.