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. 2015 Jul 31;290(38):23361–23370. doi: 10.1074/jbc.M115.658203

FIGURE 2.

FIGURE 2.

Effects of PTP-loop mutation on glucan phosphatase activity. A, model of the LSF1 PTP-loop (yellow) mutations in SEX4 (PDB code: 4PYH), showing the steric clash (red disk) with the glucan chain caused by the Asp at position 5 for LSF1. B, specific activity of SEX4, LSF2, and PTP-loop variants against soluble amylopectin. Error bars represent the ± S.D. of four replicates. Statistical comparison of wild type and mutant activities demonstrates significant differences, p < 0.001, between all constructs. C, specific activity of SEX4, LSF2, and PTP-loop variants against radiolabeled Arabidopsis starch. Error bars represent the ± S.D. of five replicates. Statistical analysis of wild type and mutant activities demonstrates significant differences, p < 0.001, between all constructs.