Effects of salts on TPQsq formation. UV-visual absorption spectra of TPQsq generated by anaerobic reaction with 2-PEA (1 mm) as the substrate were measured with 100 μm AGAO monomer in 100 mm HEPES, pH 6.8, in the absence (black solid line) and presence of various salts at a final concentration of 10 mm: NaCl (green solid line), NH4Cl (blue solid line), KCl (blue broken line), Na2SO4 (red broken line), or NaH2PO4 (red solid line).