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Steady-state kinetics analysis in the presence of NaCl. A, AGAO activity was measured at 30 °C in 50 mm HEPES (pH 6.8) in the presence of 0 (○), 10 (□), 30 (▵), or 50 (♢) mm NaCl with systematically varied 2-PEA concentrations. B, AGAO activity was measured in solutions containing 40 μm 2-PEA and 0 (○), 12.5 (□), 25 (▵), or 50 (♢) mm NaCl with systematically varied solvent O2 concentrations. Each point represents the mean ± S.E. (error bars) from two independent experiments.
