FIGURE 5.
Dependence of steady-state kinetic parameters on solvent viscosity. The ratios of kcat/Km (A) and kcat (B) in the presence and absence of viscogen at 4 °C were plotted against the relative solvent viscosity, η/η0, where zero denotes the value in the absence of viscogen. Black circles and squares, data points for glycerol and sucrose, respectively. A thin black line denotes the theoretical result for a completely diffusion-controlled process with a slope of 1.0. Error bars, S.D. (n = 2).