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Genotypes of Venus-H5N1-related reassortants and their virulence in mice. The colors indicate the origins of the gene segments (blue, WT-Venus-H5N1 virus; red, MA-Venus-H5N1 virus). MLD50 values were determined by inoculating groups of four mice with 10-fold serial dilutions containing 100 to 105 PFU of virus in a 50-μl volume and were calculated by using the method of Reed and Muench (13).
