FIG 5.

IFN-α subtypes 4, 6, 7, 10, and 17 are induced in an IFNAR-dependent manner. (A) U937 cells were infected with 1.5 HA U/ml of SeV in the presence or absence of IFNAR2-neutralizing antibody (NA) for 8 and 24 h. qRT-PCR analysis was performed measuring IFN-α4, -6, -7, -10, and -17 mRNA. Statistics were performed using one-way ANOVA and Bonferroni posttest analysis. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. The data shown are from 3 biological replicates performed in duplicate. (B) Western blot analysis measuring IRF9, IRF7, p(Y701)STAT1, p(S727)STAT1, STAT1, and actin protein expression in cells infected with 1.5 HA U/ml in the presence or absence of IFNAR2-neutralizing antibody for 4, 8, 16, and 24 h p.i. UI, uninfected. The Western blot is representative of 3 biological replicates. qRT-PCR data are shown as the percent relative expression of HPRT. The error bars indicate the standard error of the mean.