TABLE 2.
Virus excretion by black-headed gulls after one or more inoculations with LPAIV H13N2, H16N3, or both
| Group | Inoculation schedule | Virus excretion at inoculation: |
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|---|---|---|---|---|---|---|---|
| I |
II |
III |
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| Mean quantity (AUC of viral RNA) ± SEb | Median duration (days) (range)c | Mean quantity (AUC of viral RNA) ± SEb | Median duration (days) (range)c | Mean quantity (AUC of viral RNA) ± SEb | Median duration (days) (range)c | ||
| 1 | H13-H16-H16 | 151.8 ± 6.7 | 7 (5–11) | 117.6 ± 20.5 | 5 (0–9) | 51.6 ± 28.5 | 0 (0–3) |
| 2 | H16-H16-H16 | 122.3 ± 9.1 | 5.5 (4–10) | 28.5 ± 6.8 | 0 (0–3) | 13.7 ± 3.4 | 0 (0–2) |
| 3 | Sham-H16-H16 | 0 | 0 | 102.8 ± 13.4 | 6 (0–11) | 14.4 ± 7.3 | 0 (0–2) |
| 4 | Sham-sham-H16 | 0 | 0 | 0 | 0 | 122.1 ± 21.1 | 4 (0–6) |
| 5 | H16-H13a-H13 | 54.6 ± 14.1 | 3.5 (0–5) | 0 | 0 | 72.2 ± 10.3 | 0 (0–3) |
| 6 | H13-H13-H13 | 133.7 ± 11.9 | 4.5 (1–6) | 69.6 ± 8.4 | 0 (0) | 0.8 ± 0.8 | 0 (0) |
| 7 | Sham-H13a-H13 | 0 | 0 | 1.0 ± 0.6 | 2 (0–6) | 64.0 ± 21.2 | 0 (0–7) |
| 8 | Sham-sham-H13 | 0 | 0 | 0 | 0 | 117.0 ± 12.2 | 4 (0–5) |
a
The second inoculation of groups 5 and 7 was unsuccessful.
b
The quantity of virus excretion was based on the AUC for viral RNA (i.e., CT values determined by M-RT-PCR) excreted from the cloaca from days 0 to 14 postinoculation.
c
Duration of virus excretion was based on the maximum duration of infectious virus excretion based on virus culture from cloaca in days.