TABLE 3.
Antibody detection in black-headed gulls after one or more inoculations with LPAIV H13N2, H16N3, or both
| Group | Inoculation schedule | Assay | Antibody detection at inoculation: |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| I |
II |
III |
|||||||||
| Antibody titer at 0 dpi | Mean quantity of antibody production (log2 AUC) ± SEb | No. of seropositive birds/total no. of birdsc | Antibody titer at 0 dpi | Mean quantity of antibody production (log2 AUC) ± SEb | No. of seropositive birds/total no. of birdsc | Antibody titer at 0 dpi | Mean quantity of antibody production (log2 AUC) ± SEb | No. of seropositive birds/total no. of birdsc | |||
| 1 | H13-H16-H16 | H13 HI | 0 | 4.17 ± 1.38 | 4/6 | 0 | 3.90 ± 2.03 | 4/6 | 0 | 0.40 ± 0.40 | 1/4 |
| H16 HI | 0 | 1.10 ± 0.50 | 3/6 | 0 | 8.47 ± 2.69 | 5/6 | 2.33 ± 1.50 | 15.51 ± 6.03 | 4/4 | ||
| NP bELISA | 3/6 | 4/6 | 2/4 | ||||||||
| 2 | H16-H16-H16 | H13 HI | 0 | 1.10 ± 0.82 | 2/6 | 0 | 0.00 ± 0.00 | 0/6 | 0 | 3.47 ± 2.49 | 2/5 |
| H16 HI | 0 | 10.49 ± 2.54 | 6/6 | 3.65 ± 1.73 | 8.30 ± 2.38 | 5/6 | 2.18 ± 0.89 | 16.52 ± 6.78 | 4/5 | ||
| NP bELISA | 4/6 | 1/6 | 3/5 | ||||||||
| 3 | Sham-H16-H16 | H13 HI | 0 | 0.00 ± 0.00 | 0/5 | 0 | 0.00 ± 0.00 | 0/5 | 0 | 0.00 ± 0.00 | 0/5 |
| H16 HI | 0 | 0.00 ± 0.00 | 0/5 | 0 | 10.89 ± 3.24 | 4/5 | 2.10 ± 1.16 | 8.21 ± 4.60 | 3/5 | ||
| NP bELISA | 0/5 | 4/5 | 1/5 | ||||||||
| 4 | Sham-sham-H16 | H13 HI | 0 | 0.00 ± 0.00 | 0/6 | 0 | 0.00 ± 0.00 | 0/6 | 0 | 0.00 ± 0.00 | 0/6 |
| H16 HI | 0 | 0.00 ± 0.00 | 0/6 | 0 | 0.00 ± 0.00 | 0/6 | 0 | 17.45 ± 1.97 | 6/6 | ||
| NP bELISA | 0/6 | 0/6 | 5/6 | ||||||||
| 5 | H16-H13a-H13 | H13 HI | 0 | 0.00 ± 0.00 | 0/5 | 0 | 0.83 ± 0.83 | 1/5 | 0 | 10.48 ± 3.61 | 4/5 |
| H16 HI | 0 | 6.12 ± 2.65 | 3/5 | 1.98 ± 0.89 | 3.50 ± 1.33 | 4/5 | 0 | 11.43 ± 5.03 | 3/5 | ||
| NP bELISA | 3/5 | 0/5 | 4/5 | ||||||||
| 6 | H13-H13-H13 | H13 HI | 0 | 1.67 ± 1.17 | 2/6 | 0 | 3.86 ± 2.19 | 4/6 | 0 | 7.37 ± 4.31 | 4/5 |
| H16 HI | 0 | 0.83 ± 0.54 | 2/6 | 0 | 1.00 ± 1.00 | 1/6 | 0 | 5.88 ± 2.74 | 3/5 | ||
| NP bELISA | 4/6 | 2/6 | 3/5 | ||||||||
| 7 | Sham-H13a-H13 | H13 HI | 0 | 0.00 ± 0.00 | 0/6 | 0 | 0.43 ± 0.43 | 1/6 | 0 | 4.11 ± 1.53 | 4/5 |
| H16 HI | 0 | 0.00 ± 0.00 | 0/6 | 0 | 0.00 ± 0.00 | 0/6 | 0 | 0.60 ± 0.60 | 1/5 | ||
| NP bELISA | 0/6 | 0/6 | 2/5 | ||||||||
| 8 | Sham-sham-H13 | H13 HI | 0 | 0.00 ± 0.00 | 0/6 | 0 | 0.00 ± 0.00 | 0/6 | 0 | 2.20 ± 1.02 | 3/5 |
| H16 HI | 0 | 0.00 ± 0.00 | 0/6 | 0 | 0.00 ± 0.00 | 0/6 | 0 | 1.03 ± 0.43 | 3/5 | ||
| NP bELISA | 1/6 | 0/6 | 4/5 | ||||||||
a
The second inoculation of groups 5 and 7 was unsuccessful.
b
Antibody production is based on the AUC on a log2 scale between days 0 and 28 postinoculation.
c
Total number of birds that seroconverted between days 0 and 28 postinoculation.