TABLE 4.
Year-round antibody detection after one or more inoculations of black-headed gulls with LPAIV H13N2, H16N3, or both
| Group | Inoculation schedule | Assay | No. of seropositive birds/total no. of birds after inoculationb: |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| I (0 mo) | II at mo: |
III at mo: |
||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | ||||
| 1 | H13-H16-H16 | H13 HI | 0/6 | 0/6 | 2/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 |
| H16 HI | 0/6 | 0/6 | 4/6 | 1/6 | 3/6 | 2/6 | 2/6 | 1/4 | 1/4 | 1/4 | 1/4 | 1/4 | 1/4 | 3/4 | 2/4 | 2/4 | ||
| NP bELISA | 0/6 | 0/6 | 1/6 | 1/6 | 0/6 | 0/6 | 0/6 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 1/4 | 1/4 | 1/4 | ||
| 2 | H16-H16-H16 | H13 HI | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 |
| H16 HI | 0/6 | 4/6 | 4/6 | 3/6 | 4/6 | 3/6 | 3/6 | 3/6 | 2/6 | 3/6 | 3/6 | 3/6 | 2/5 | 3/5 | 2/5 | 2/5 | ||
| NP bELISA | 0/6 | 1/6 | 1/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | ||
| 3 | Sham-H16-H16 | H13 HI | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| H16 HI | 0/5 | 0/5 | 3/5 | 2/5 | 2/5 | 2/5 | 2/5 | 2/5 | 2/5 | 2/5 | 2/5 | 2/5 | 1/5 | 3/5 | 3/5 | 3/5 | ||
| NP bELISA | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 1/5 | 1/5 | 1/5 | ||
| 4 | Sham-sham-H16 | H13 HI | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/4 |
| H16 HI | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 6/6 | 2/5 | 2/4 | ||
| NP bELISA | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/4 | ||
| 5 | H16-H13a-H13 | H13 HI | 0/5 | 0/5 | 1/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 4/5 | 3/5 | 2/5 |
| H16 HI | 0/5 | 1/5 | 1/5 | 1/5 | 2/5 | 1/5 | 2/5 | 2/5 | 1/5 | 2/5 | 2/5 | 1/5 | 0/5 | 3/5 | 3/5 | 3/5 | ||
| NP bELISA | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 3/5 | 1/5 | 1/5 | ||
| 6 | H13-H13-H13 | H13 HI | 0/6 | 0/6 | 1/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | 1/5 | 1/5 | 1/5 |
| H16 HI | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | 2/5 | 1/5 | 1/5 | ||
| NP bELISA | 0/6 | 0/6 | 0/6 | 0/6 | 1/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | 2/5 | 1/5 | 0/5 | ||
| 7 | Sham-H13a-H13 | H13 HI | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 1/5 | 0/5 | 0/5 |
| H16 HI | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | ||
| NP bELISA | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | ||
| 8 | Sham-sham-H13 | H13 HI | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| H16 HI | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | ||
| NP bELISA | 0/6 | 0/6 | 0/6 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | ||
a
The second inoculation of groups 5 and 7 was unsuccessful.
b
Boldface type indicates detection of seropositivity.