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. 2015 Sep 2;89(22):11507–11522. doi: 10.1128/JVI.01765-15

TABLE 4.

Year-round antibody detection after one or more inoculations of black-headed gulls with LPAIV H13N2, H16N3, or both

Group Inoculation schedule Assay No. of seropositive birds/total no. of birds after inoculationb:
I (0 mo) II at mo:
III at mo:
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
1 H13-H16-H16 H13 HI 0/6 0/6 2/6 0/6 0/6 0/6 0/6 0/4 0/4 0/4 0/4 0/4 0/4 0/4 0/4 0/4
H16 HI 0/6 0/6 4/6 1/6 3/6 2/6 2/6 1/4 1/4 1/4 1/4 1/4 1/4 3/4 2/4 2/4
NP bELISA 0/6 0/6 1/6 1/6 0/6 0/6 0/6 0/4 0/4 0/4 0/4 0/4 0/4 1/4 1/4 1/4
2 H16-H16-H16 H13 HI 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 0/5 0/5 0/5
H16 HI 0/6 4/6 4/6 3/6 4/6 3/6 3/6 3/6 2/6 3/6 3/6 3/6 2/5 3/5 2/5 2/5
NP bELISA 0/6 1/6 1/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 0/5 0/5 0/5
3 Sham-H16-H16 H13 HI 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5
H16 HI 0/5 0/5 3/5 2/5 2/5 2/5 2/5 2/5 2/5 2/5 2/5 2/5 1/5 3/5 3/5 3/5
NP bELISA 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 1/5 1/5 1/5
4 Sham-sham-H16 H13 HI 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 0/4
H16 HI 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 6/6 2/5 2/4
NP bELISA 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 0/4
5 H16-H13a-H13 H13 HI 0/5 0/5 1/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 4/5 3/5 2/5
H16 HI 0/5 1/5 1/5 1/5 2/5 1/5 2/5 2/5 1/5 2/5 2/5 1/5 0/5 3/5 3/5 3/5
NP bELISA 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 3/5 1/5 1/5
6 H13-H13-H13 H13 HI 0/6 0/6 1/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 0/5 0/5 0/5 1/5 1/5 1/5
H16 HI 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 0/5 0/5 0/5 2/5 1/5 1/5
NP bELISA 0/6 0/6 0/6 0/6 1/6 0/6 0/6 0/6 0/6 0/5 0/5 0/5 0/5 2/5 1/5 0/5
7 Sham-H13a-H13 H13 HI 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 1/5 0/5 0/5
H16 HI 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 0/5 0/5 0/5
NP bELISA 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/5 0/5 0/5 0/5
8 Sham-sham-H13 H13 HI 0/6 0/6 0/6 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5
H16 HI 0/6 0/6 0/6 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5
NP bELISA 0/6 0/6 0/6 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5 0/5
a

The second inoculation of groups 5 and 7 was unsuccessful.

b

Boldface type indicates detection of seropositivity.