FIG 4.

Secretion of IFN-γ after antigen-specific restimulation of splenocytes. (A to C) IFN-γ ELISpot analysis results with splenocytes of mice vaccinated on days 0 and 28 with indicated viruses, isolated 4 days after boost immunization and after coculture with JAWSII (A), DC2.4 (B), or DC3.2 (C) dendritic cell lines transgenic for MERS-S or untransduced controls (NC). (D) To analyze cellular responses directed against MV, splenocytes were stimulated with 10 μg/ml MV bulk antigens (MV-N) or left unstimulated (unst.). The reactivity of splenocytes was confirmed by ConA treatment (10 μg/ml). Presented are means and standard deviation per group (n = 6). ns, not significant; *, P < 0.05; **, P < 0.01.