FIG 1.

Preliminary characterization of a UL36-null HSV strain. (A) Single-step growth curves of the UL36-null HSV1-GS2491 on Vero and complementing HS30 cells. Vero (V) or HS30 (H) cells were infected with HSV-1 strain K26GFP (K26) or HSV1-GS2491 (GS) at an MOI of 10. After 1 h, they were acid washed to inactivate unpenetrated virus, and then cells were harvested immediately or after an additional 12 or 18 h at 37°C. Cell extracts were prepared, and their titers were determined on HS30 cells. Plotted values and error bars represent the means and standard deviations from the means. (B) Vero cells were infected with K26GFP or HSV1-GS2491 exactly as described for panel A. After 16 h of infection, total cell extracts were prepared and then subjected to SDS-PAGE and immunoblotted for UL36p. (C) PNS and membrane-associated fractions were prepared from K26GFP- or HSV1-GS2491-infected Vero or HS30 cells. They were then subjected to SDS-PAGE and immunoblotted for the major capsid protein VP5 and for tegument protein vhs, VP16, VP22, or UL37p (as indicated on the left). In panels B and C, the lanes are labeled at the top according to the virus strain used and the cell line infected, as described for panel A.