TABLE 1.
Quantitation of DiD lipophilic dye-stained GFP-capsid-associated particles in the PNS of HSV-infected cellsa
| Virus-cell (concn of DiD [μM])b | Mean no. (± SD) of particles per field |
DiD-positive green particles as % of total | |
|---|---|---|---|
| Total green | DiD-positive green | ||
| K26-V (0) | 1,108 ± 178 | 13 ± 3 | 1.2 ± 0.3 |
| K26-V (10) | 1,211 ± 286 | 226 ± 65 | 18.7 ± 4.7 |
| K26-H (0) | 1,131 ± 96 | 14 ± 5 | 1.2 ± 0.4 |
| K26-H (10) | 1,195 ± 143 | 257 ± 27 | 21.5 ± 2.9 |
| GS-V (0) | 1,062 ± 125 | 15 ± 4 | 1.4 ± 0.4 |
| GS-V (10) | 1,073 ± 240 | 228 ± 49 | 21.2 ± 5.6 |
| GS-H (0) | 1,085 ± 102 | 14 ± 4 | 1.3 ± 0.4 |
| GS-H (10) | 1,086 ± 160 | 216 ± 46 | 19.9 ± 4.5 |
a
For each concentration of dye, counting was done in 12 independent microscopic fields from K26-V and K26-H and in 11 fields from GS-V and GS-H to determine the mean particle number per field.
b
V, Vero cells; H, HS30 cells; K26, HSV-1 strain K26GFP; GS, HSV1-GS2491.