TABLE 2.
Quantitation of DilC18(5)-DS (Dil) lipophilic dye-stained GFP-capsid-associated particles in the PNS of HSV-infected cellsa
| Virus-cell (concn of Dil [μM])b | Mean no. (± SD) of particles per field |
Dil-positive green particles as % of total | |
|---|---|---|---|
| Total green | Dil-positive green | ||
| K26-V (0) | 1,189 ± 64 | 14 ± 3 | 1.2 ± 0.3 |
| K26-V (10) | 1,194 ± 86 | 333 ± 52 | 27.9 ± 3.5 |
| K26-H (0) | 1,220 ± 77 | 16 ± 4 | 1.3 ± 0.4 |
| K26-H (10) | 1,314 ± 120 | 389 ± 45 | 29.6 ± 3.1 |
| GS-V (0) | 1,195 ± 23 | 18 ± 2 | 1.5 ± 0.2 |
| GS-V (10) | 1,254 ± 112 | 354 ± 36 | 28.2 ± 2.8 |
| GS-H (0) | 1,221 ± 73 | 17 ± 4 | 1.4 ± 0.3 |
| GS-H (10) | 1,221 ± 79 | 336 ± 48 | 27.5 ± 3.4 |
a
For each concentration of dye, counting was done in five independent microscopic fields from K26-V and K26-H and in seven fields from GS-V and GS-H to determine the mean particle number per field.
b
V, Vero cells; H, HS30 cells; K26, HSV-1 strain K26GFP; GS, HSV1-GS2491.