FIG 5.

Artificial siRNA (asiRNA)-mediated knockdown of BmCPV polyhedrin presented as the percent expression of the control (C, nonspecific asiRNA). c1 to c4, m1 to m4, and h1 to h4 asiRNA map to the cold, moderate, and hot spots of BmCPV segment 10, respectively. A representative Western blot showing knockdown of polyhedrin by moderate and cold spot asiRNA is shown in the inset image. Specific proteins detected by Myc antibody were FHV-cp1-MycHis (*; 45 kDa; transfection normalization protein) and BmCPV-polyhedrin-MycHis (**, 30 kDa).