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. 2015 Sep 2;89(22):11473–11486. doi: 10.1128/JVI.01695-15

FIG 6.

FIG 6

Structured regions in transcripts of S10 can function as an additional source of vsRNAs. (A) Predicted secondary structure of the positive strand of S10 (or the polyhedrin transcript) using RNAfold. The detail depicts the 82-nt stem-loop that overlaps with a hot spot for (+)-vsRNA production. (B) Hairpin (H)-mediated knockdown of BmCPV polyhedrin expression in Western blot compared to mutated hairpin (MutH). Hi5 cells were cotransfected with MycHis-tagged BmCPV-polyhedrin and Myc-tagged FHV-cp1 (for normalization) expression constructs, together with either H RNA corresponding to the predicted 82-nt stem-loop or MutH RNA. A representative Western blot showing knockdown of polyhedrin by H RNA is shown at the top of the panel. Specific proteins detected by Myc antibody were FHV-cp1-MycHis (*; 45 kDa; transfection normalization protein) and BmCPV-polyhedrin-MycHis (**, 30 kDa).