FIG 2.

Cell-type-specific uptake of MGBG in primary human blood cells. Different cell types prepared from human PBMCs or white blood cells were cultured with 1.4 μM MGBG for 24 h. Cell lysates were prepared from the cell for quantification of MGBG and cellular proteins. MGBG uptake was either normalized to the amount of cellular proteins (A) or converted to intracellular MGBG concentrations by using published mean cell volumes (B). PMN, polymorphonuclear leukocytes. The mass peaks for MGBG in the resting T cells and granulocytes are below the analytical detection limit (BDL), which is 10 nM in the lysates, ∼120 pmol/mg protein for the resting T cells, and 70 pmol/mg protein for the granulocytes. The graphs show means and SEMs (n ≥ 3).